%0 Journal Article
%T The usefulness of immunohistochemistry in tissue microarrays of Human Papillomavirus negative adenocarcinoma of the uterine cervix
%A Michael Odida
%A Belen Lloveras
%A Nuria Guimera
%A Elisabete Weiderpass
%J BMC Research Notes
%D 2010
%I BioMed Central
%R 10.1186/1756-0500-3-54
%X Five commercially available antibodies were tested in tissue array sections immunostained utilizing the avidin-biotin (AB) technique. In 26 ADC samples, HPV was detected in 13, p16 in 15 (8 in HPV positive and 7 in HPV negative), CEA in 12, vimentin in 6, ER in 0, and PR in 2. Among the 13/25 HPV negative ADC samples, five were positive for CEA suggesting endocervical origin, and three were vimentin positive (one had a mucinous endocervical histological pattern and two were ADC, not otherwise specified, most likely of endometrial origin).The immunoprofiles of ADC with the antibodies studied are rather nonspecific. By using immunohistochemistry in 13 HPV negative ADC, endocervical tumour origin was suspected in five CEA positive cases while two out of three vimentin positive samples were probably of endometrial origin, suggesting that CEA and vimentin may be valuable in distinguishing HPV negative cervical adenocarcinomas from endometrial adenocarcinomas.Although the role of HPV in cervical adenocarcinoma appears well established [1-3], the nature of adenocarcinoma arising from the cervix may be difficult to define due to the overlapping morphological features between cervical adenocarcinomas and endometrial adenocarcinomas. While endocervical adenocarcinomas express p16 and CEA [4] and whereas endometrial adenocarcinomas usually showed vimentin and hormonal receptors [5], the results may be inconclusive. However, CEA has been reported to be expressed in both cervical and endometrial adenocarcinomas [6], while the expression of vimentin was noted to be weak and focal in many endometrial adenocarcinomas [5]. An alternative view is that some expression of these markers may reflect differentiation (mucinous versus endometrioid) compared to the histogenetic site of origin (endometrial versus cervical) [7]. In order to resolve the problem, HPV testing has been used in attempts to distinguish these tumours [8]. Extending this approach, a recent study evaluated the use of H
%U http://www.biomedcentral.com/1756-0500/3/54