%0 Journal Article
%T Myostatin genotype regulates muscle-specific miRNA expression in mouse pectoralis muscle
%A Satyanarayana Rachagani
%A Ye Cheng
%A James M Reecy
%J BMC Research Notes
%D 2010
%I BioMed Central
%R 10.1186/1756-0500-3-297
%X Loss of functional Myostatin resulted in a significant increase (p < .001) in miR-1, miR-133a, miR-133b, and miR-206 expression. In contrast, Myostatin genotype had no effect (P > .2) on miR-24 expression level. Myostatin genotype did not affect the expression level of MyoD or Myogenin (P > 0.5).Myostatin may regulates the expression of miRNAs such as miR-133a, miR-133b, miR-1, and miR-206 in skeletal muscle as it has been observed that the expression of those miRNAs are significantly higher in myostatin null mice compared to wild type and heterozygous mice. In contrast, expression of myogenic factors such as MyoD or Myogenin has not been affected by myostatin in the muscle tissue.miRNAs have generated strong interest among researchers in different fields to understand their biosynthesis, mechanism of action, and identification of their targets. First discovered in the caenorhabditis elegans as small temporal RNAs required for proper developmental timing, miRNA are a family of highly conserved, non-coding, 22 nucleotide [1] double-stranded RNA products that post-transcriptionally regulate gene action[1-4].Sampere et al. (2004) first identified the expression of the muscle specific miRNas such as miR-1, -133a and -206[5]. Their expression was highly enriched in both human and mouse heart and skeletal muscle. Subsequently, several miRNA expression profiling studies have consistently shown miR-1, -133a and -206 to be muscle specific[6-11]. These muscle specific expressions are evolutionarily conserved among animals[12]. Over expression of miR-1 and miR-133 during the in-vitro development of embryoid bodies from mouse embryonic stem cells demonstrated that distinct steps in muscle development are specified by cooperative and opposing interactions between miR-1 and miR-133.Given that miRNAs have been shown to play roles in the regulation of satellite cell proliferation and differentiation, the current study was carried out to evaluate the effect of loss of Myostatin func
%U http://www.biomedcentral.com/1756-0500/3/297