%0 Journal Article
%T Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16
%A ANJA MERYANDINI
%A TRIO HENDARWIN
%A FAHRRUROZI
%A ALINA AKHDIYA
%J Biodiversitas
%D 2009
%I MBI & UNS Solo
%X Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2   hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1,98 mg/mL and 523 ¦̀mol/minute/mg, respectively.
%K xylanase
%K xylooligosaccharide
%K arabinofuranosidase
%K Streptomyces
%U http://biodiversitas.mipa.uns.ac.id/D/D1003/D100302.pdf