%0 Journal Article
%T KINETIC STUDY OF PALMITIC ACID ESTERIFICATION CATALYZED BY Rhizopus oryzae RESTING CELLS
%A M谷NDEZ JONH J
%A RAMON CANELA
%A TORRES MERCˋˋ
%J Acta Biol車gica Colombiana
%D 2009
%I Universidad Nacional de Colombia
%X ABSTRACT In the present study, a kinetic model for the biocatalytic synthesis of esters using Rhizopus oryzae resting cells is proposed. The kinetic study has been made in a range of 30-50 ∼C and atmospheric pressure. The Influence of operating variables, water content, pH, amount of mycelium was studied. Different values of temperature, initial mycelium concentration and acid/alcohol molar ratio were tested. Initial rates were estimated from the slope of the concentration of palmitic acid, or their corresponding ester at conversions of less than 10%, versus time and reported as mmol l-1 min -1. The values of kinetic constants were computed using the freeware program SIMFIT (http:www.simfit.man.ac.uk). Key words: bound lipase, esterification, fungal resting cells, Rhizopus oryzae, palmitic acid, propanol. RESUMEN En el presente estudio, un modelo cin谷tico para la s赤ntesis de esteres usando Rhizopus oryzae resting cells es propuesto. El estudio cin谷tico fue realizado en un rango de temperatura de 30-50 oC a presi車n atmosf谷rica reducida. La influencia de las variables de operaci車n tales como temperatura, pH y contenido de agua fueron estudiadas. Diferentes valores de concentraci車n de micelio y relaci車n molar de 芍cido/alcohol son ensayadas, Las velocidades in赤ciales se estimaron de la curva de concentraci車n de acido palm赤tico, y su correspondiente conversi車n a ester en menos del 10%, frente a tiempo y reportadas en mmol I-1 min -1. Los valores de las constantes cin谷ticas fueron calculados usando el programa freeware SIMFIT (http:www.simfit.man.ac.uk). Palabras clave: Lipasas, esterificaci車n, resting cells, Rhizopus oryzae, acido palm赤tico, propanol.
%U http://revistas.unal.edu.co/index.php/actabiol/article/view/9776