%0 Journal Article
%T Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
%A Andrea P. R odriguez
%A Hidetsugu Tsujigiwa
%A Mehmet Gunduz
%A Beyhan Cengiz
%J Biocell
%D 2009
%I Universidad Nacional de Cuyo
%X Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
%K MDPC-23 cells
%K KUSA/A1 cells
%K Diffusion chamber
%K RT-PCR analysis
%K Immunohistochemical analysis
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100005