%0 Journal Article
%T Rumen ciliate protozoa in sheep raising in the caatinga region of Pernambuco State, Brazil Popula  o de protozo¨¢rios ciliados no r¨²men de ovinos criados na caatinga de Pernambuco
%A Daniele Silva de Matos
%A Adriana Guim
%A £¿ngela Maria Vieira Batista
%A M¨¦rcia Virg¨ªnia Ferreira dos Santos
%J Revista Brasileira de Sa¨²de e Produ£¿£¿o Animal
%D 2008
%I Universidade Federal da Bahia
%X This work sought to determine the number and genus of rumen ciliate protozoa in caatinga sheep raising, before and four hours after feeding them. There were collected 10 mL of ruminal fluid which was fixed in 10 mL of 10% formalin. After counting and identification of genera, the variables were submitted to variance analysis, the means compared by Tukey test and accomplished of simple correlation test between protozoa and chemical composition diet. Among found genera there was predominance of Entodinium (around 90%). Holotricha subclass only Isotricha genus was registered which represented 1.94% of population. The percentage of protozoa only varied (P <0.05) for Eodinium genus. It¡¯s highest variation was showed in May 2005. Related to the collection schedule, only the pH, the medium number of Entodinium gender and total number of protozoa presented significant difference, being larger before feeding the animals. Other genera also presented the same behavior, thus reflecting the stability of ruminal environment for animals without access to night feeding. The protozoa of Entodiniomorphid subclass present high positive correlation with level of total digestible nutrients and crude protein of the diet. This reflects the necessity, for microorganism, of energy and protein for synthesis (cellular division) and storage. Objetivou-se com este trabalho determinar o n¨²mero e g¨ºnero de protozo¨¢rios ciliados no r¨²men de ovinos criados na caatinga antes da alimenta  o e quatro horas ap¨®s. Foram coletados 10 mL de fluido ruminal, fixados em 10 mL de formalina a 10 %. Ap¨®s contagem e identifica  o dos g¨ºneros, as vari¨¢veis foram submetidas ¨¤ an¨¢lise de variancia, as m¨¦dias comparadas pelo teste de Tukey, sendo, ainda, realizado o teste de correla  o simples entre protozo¨¢rios e a composi  o qu¨ªmica da dieta. Dentre os g¨ºneros encontrados, houve predominancia do g¨ºnero Entodinium (em torno de 90%). Da subclasse Holotricha, foi registrado somente o g¨ºnero Isotricha que representou em m¨¦dia 1,94% da popula  o. A percentagem de protozo¨¢rios s¨® variou (P<0,05) para o g¨ºnero Eodinium, sendo maior em maio de 2005. Com rela  o ao hor¨¢rio de coleta, somente o pH, a concentra  o m¨¦dia do g¨ºnero Entodinium e o n¨²mero total de protozo¨¢rios apresentaram diferen a significativa, sendo maior antes da alimenta  o. Os protozo¨¢rios da subclasse Entodiniomorpha apresentam correla  o positiva (P<0,05) com o n¨ªvel de nutrientes digest¨ªveis totais e a prote¨ªna bruta da dieta, o que reflete a necessidade, pelo microrganismo, de energia e prote¨ªna para a s¨ªntese (divis o celular) e armazena
%U http://revistas.ufba.br/index.php/rbspa/article/view/933