%0 Journal Article
%T Thidiazuron induced plant regeneration in callus culture of triploid banana (Musa sp.) ¡®Gros Michel¡¯, AAA group
%A Amornwat Srangsam
%A Kamnoon Kanchanapoom
%J Songklanakarin Journal of Science and Technology
%D 2003
%I Songkla University
%X Yellow friable calluses of banana (Musa sp.) ¡®Gros Michel¡¯, AAA group were initiated from in vitro shoot buds cultured on Murashige and Skoog (MS) solid medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and coconut water (CW). Small spherical, compact calluses were formed after friable calluses were transferred to half-MS liquid media supplemented with 1.5 mg/l 2, 4-D or 1.5 mg/l 2, 4-D in combination with 5% CW or without 2,4-D and CW. However, no shoots were produced from these media. Embryogenic calluses were induced followed by subculture the spherical, compact calluses to half-MS solid medium in the presence of thidiazuron (TDZ). These embryogenic calluses gave rise to shoots on MS germination medium containing 2.0 mg/l ¦Á-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzyladenine (BA). Complete banana plants were recovered on MS maturation medium containing 0.5 mg/l BA and 0.2% activated charcoal. Morphological observation of plantlets did not show any abnormality.
%K banana
%K callus culture
%K ¡®Gros Michel¡¯ AAA group
%K organogenesis
%K thidiazuron
%U http://www.sjst.psu.ac.th/journal/25-6-pdf/01callus_banana.pdf