%0 Journal Article
%T Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
%A Kyle S Hubbard
%A Ian M Gut
%A Stephen M Scheeler
%A Megan E Lyman
%A Patrick M McNutt
%J BMC Research Notes
%D 2012
%I BioMed Central
%R 10.1186/1756-0500-5-437
%X SYTO 13 is acutely neurotoxic and when used in longitudinal studies, masked end-stage morphologic evidence of apoptotic cell death. In contrast, a single application of Hoechst evoked no evidence of toxicity over a 6 h period, and was consistent with end-point characterizations of cell viability and nuclear morphology. For longitudinal characterization of acute cell death, Hoechst is a superior option.
%U http://www.biomedcentral.com/1756-0500/5/437/abstract