%0 Journal Article
%T Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells
%A Andreas Wemh£¿ner
%A Paul Jennings
%A Thomas Haller
%A Mario R¨¹diger
%A Georg Simbruner
%J BMC Pulmonary Medicine
%D 2011
%I BioMed Central
%R 10.1186/1471-2466-11-11
%X The aim of this study was to investigate the effects of two commercially available surfactant preparations on ATII cell viability and DNA synthesis.Curosurf£¿ and Alveofact£¿ were applied to two ATII cell lines (human A549 and mouse iMATII cells) and to primary rat ATII cells for periods of up to 24 h. Cell viability was measured using the redox indicator resazurin and DNA synthesis was measured using BrdU incorporation.Curosurf£¿ resulted in slightly decreased cell viability in all cell culture models. However, DNA synthesis was increased in A549 and rat ATII cells but decreased in iMATII cells. Alveofact£¿ exhibited the opposite effects on A549 cells and had very mild effects on the other two cell models.This study showed that commercially available exogenous surfactants used to treat preterm infants with RDS can have profound effects on cell viability and DNA synthesis.Respiratory Distress Syndrome (RDS) is caused by surfactant deficiency and is a leading cause of mortality and morbidity in preterm newborns. Some infants develop the chronic lung disease, bronchopulmonary dysplasia (BPD), which is a major cause of long term morbidity [1]. BPD, as described by Northway [2], is mainly caused by a pulmonary injury associated with mechanical ventilation [3]. The subsequent histological correlate is characterised by cellular proliferation and fibrosis at the alveolar level [4,5]. Another entity of chronic lung disease is "New BPD" which occurs even without any mechanical ventilation. New BPD is characterized by abnormal development of alveolar and capillary structures [5].A key finding in both forms of chronic lung disease is an abnormal cellular proliferation in cells of the alveolus. Type II pneumocytes (ATII cells) are one of the major alveolar cell types and function to synthesise, secrete and recycle surfactant, and are also involved in immunological processes. In response to lung injury, ATII cells proliferate and differentiate into type I cells to maintain the alveo
%U http://www.biomedcentral.com/1471-2466/11/11