%0 Journal Article
%T Canine visceral leishmaniasis and Chagas disease among dogs in Aragua赤na, Tocantins Leishmaniose visceral canina e doen a de Chagas em c es de Aragua赤na, Tocantins
%A Arielle Nunes Morais
%A Marlos Gonˋalves Sousa
%A Luciana Regina Meireles
%A Norival Kesper Jr.
%J Revista Brasileira de Parasitologia Veterin芍ria
%D 2013
%I Col谷gio Brasileiro de Parasitologia Veterinaria
%X The present study analyzed serum samples from 111 male and female dogs of various ages from the municipality of Aragua赤na in the State of Tocantins, Brazil. Serological diagnosis of canine visceral leishmaniasis (CVL) was initially performed at the Central Laboratory (Laborat車rio Central 每 LACEN) of Aragua赤na, resulting in 61 positive samples by an indirect immunofluorescence assay (IIFA) (≡1:40) and 50 non-reactive samples. The same samples were analyzed at the S o Paulo Institute of Tropical Medicine (Instituto de Medicina Tropical de S o Paulo 每 IMTSP) by an enzyme-linked-immunosorbent assay (ELISA), resulting in 57 positive samples (51.35%) and 54 negative samples (48.64%). The Kappa coefficient of agreement between the tests was 0.74. The serum samples were also subjected to a diagnostic assay for Trypanosoma cruzi (Trypomastigote Excreted/Secreted Antigens -TESA-blot) that detected five suspect animals; three of those animals were positive for leishmaniasis by ELISA but negative by IIFA. These findings suggest that the canine population of Aragua赤na may be simultaneously infected with Leishmania chagasi and T. cruzi. The results obtained demonstrate the difficulty of using serology to detect CVL, thus emphasizing the necessity for a reference test to diagnose CVL, particularly in regions where the infection is endemic. Neste estudo foram analisadas amostras de soros de 111 c es machos e f那meas, de idades variadas, provenientes do munic赤pio de Aragua赤na, estado do Tocantins, Brasil. O diagn車stico sorol車gico para leishmaniose visceral canina foi realizado, inicialmente, no Laborat車rio Central (LACEN) de Aragua赤na, resultando em 61 amostras positivas na Rea  o de Imunofluoresc那ncia Indireta - RIFI (≡1:40) e 50 amostras n o reativas. As mesmas amostras foram analisadas no Instituto de Medicina Tropical de S o Paulo (IMTSP) pelo Enzyme-Linked-Immunosorbent Assay (ELISA), sendo 57 amostras positivas (51,35%) e 54 amostras negativas (48,64%), com coeficiente de concordancia entre os testes (Kappa = 0,74). Os soros foram submetidos tamb谷m a um teste de diagn車stico para Trypanosoma cruzi (Trypomastigote Excreted/Secreted Antigens -TESA-blot), o qual detectou cinco animais suspeitos, dos quais tr那s foram positivos para leishmaniose no ELISA, mas negativos na RIFI. Estas observa  es mostram que a popula  o canina de Aragua赤na pode tamb谷m estar infectada simultaneamente com Leishmania chagasi e T. cruzi. Estes resultados mostram a dificuldade da sorologia na detec  o da Leishmaniose Visceral Canina (LVC), refor ando a necessidade de um teste de refer那ncia par
%K Leishmaniose visceral canina
%K doen a de Chagas
%K RIFI
%K ELISA
%K TESA-blot
%K Canine visceral leishmaniasis
%K Chagas disease
%K IIFA
%K ELISA
%K TESA-blot
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013005000156