%0 Journal Article
%T KARAKTERISASI KITIN DEASETILASE TERMOSTABIL ISOLAT BAKTERI ASAL PANCURAN TUJUH, BATURADEN, JAWA TENGAH [Characterization of Thermostable Chitin Deacetylase from Bacteria Strain Pancuran Tujuh, Baturaden, Center of Java]
%A Siswa Setyahadi1)
%A Tatit K Bunasor2)
%A Deuxianto Hendarsyah3)
%J Jurnal Teknologi dan Industri Pangan
%D 2006
%I Indonesian Food Technologists Association (IAFT)
%X Chitin deacetylase is the enzymes that has important role in converting chitin to chitosan. In nature, chitin is the second most abundant natural biopolymer after cellulose. Generally, chitin easily obtained from outer shell of crustaceans, arthropods, and also detectable on cell wall of some type of fungal (Zygomycetes). The chitin deacetylase was isolated from Bacillus sp PT2-3. It was found that the highest specific activity was attained at pH 8 60ˇăC. The addition of 5 mM Zn2+ and 5 mM Mn2+ increased the specific activity of the enzyme, 4.39% and 7.8%, respectively, and the increase was only 2.19% when the addition was 2 mM Mn2+. On the contrary the addition of Ca2+, Mg2+ and Fe2+ decrease the specific activity 46.83%, 41.22% and 47.32%, respectively. The enzyme activity was relatively stable at 60ˇăC for 60 minutes, while lengthen the time to 90 minutes, decreased the activity 15.05 %, and the decrease was 26.13% at temperature of 70ˇăC for 180 minutes.
%K chitin deacetylase
%K 
%K Bacillus
%K thermostabil
%U http://journal.ipb.ac.id/index.php/jtip/article/view/411