%0 Journal Article
%T Improved human disease candidate gene prioritization using mouse phenotype
%A Jing Chen
%A Huan Xu
%A Bruce J Aronow
%A Anil G Jegga
%J BMC Bioinformatics
%D 2007
%I BioMed Central
%R 10.1186/1471-2105-8-392
%X Extending on an earlier hypothesis that the majority of genes that impact or cause disease share membership in any of several functional relationships we, for the first time, show the utility of mouse phenotype data in human disease gene prioritization. We study the effect of different data integration methods, and based on the validation studies, we show that our approach, ToppGene http://toppgene.cchmc.org webcite, outperforms two of the existing candidate gene prioritization methods, SUSPECTS and ENDEAVOUR.The incorporation of phenotype information for mouse orthologs of human genes greatly improves the human disease candidate gene analysis and prioritization.Although the availability of complete genome sequences and the wealth of large-scale biological data sets opened up unprecedented opportunities to elucidate the genetic basis of rare and common human diseases [1], comprehending the underlying pathophysiological mechanisms continues to be challenging. Majority of the common diseases are genetically intricate, polygenic and multifactorial, and frequently manifest as different clinical phenotypes. Additionally, these complex conditions are often triggered by an interaction of genetic, environmental, and physiological factors, making it difficult for researchers to narrow their focus to a single or few genes. High-throughput genome-wide studies like linkage analysis and gene expression profiling although useful for classification and characterization do not provide sufficient information to identify specific disease causal genes. Both of these approaches typically result in hundreds of potential candidate genes, failing to help the researchers in reducing the target genes to a manageable number for further validation.Functional enrichment approaches [2-4] focusing on gene sets that share common biological function, chromosomal location, or regulation although successful in identifying enriched biological themes are not suitable for gene prioritization. To overco
%U http://www.biomedcentral.com/1471-2105/8/392