%0 Journal Article
%T Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique
%A Raheleh Rezaei Araghi
%A Carsten C. Mahrenholz
%A Rudolf Volkmer
%A Beate Koksch
%J Beilstein Journal of Organic Chemistry
%D 2012
%I 
%R 10.3762/bjoc.8.71
%X We screened a randomized library and identified natural peptides that bound selectively to a chimeric peptide containing ¦Á-, ¦Â- and ¦Ã-amino acids. The SPOT arrays provide a means for the systematic study of the possible interaction space accessible to the ¦Á¦Â¦Ã-chimera. The mutational analysis reveals the dependence of the binding affinities of ¦Á-peptides to the ¦Á¦Â¦Ã-chimera, on the hydrophobicity and bulkiness of the side chains at the corresponding hydrophobic interface. The stability of the resulting heteroassemblies was further confirmed in solution by CD and thermal denaturation.
%K ¦Â- and ¦Ã-amino acids
%K coiled coil
%K foldamer
%K screening libraries
%K SPOT technique
%U http://dx.doi.org/10.3762/bjoc.8.71