%0 Journal Article
%T In Vitro Effect of a-Tocopherol, Ascorbic Acid and Lycopene on Low Density Lipoprotein Glycation
%A Mohammad Ali Ghaffari
%A Samad Mojab
%J Iranian Journal of Pharmaceutical Research
%D 2007
%I School of Pharmacy, Shahid Beheshti University of Medical Sciences
%X Nonenzymatic glycation of low density lipoprotein (LDL) is a reaction of glucose and other reducing sugars with apolipoprotein B100 (apo-B100) lysine residues. In diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. The objective of this study was to investigate in vitro effects of ¦Á-tocopherol, ascorbic acid and lycopene on LDL glycation. LDL was isolated from EDTA-plasma by ultracentrifugation using a single step discontinuous gradient. LDL and glucose were incubated without and with different concentrations of lycopene, ascorbic acid and ¦Á-tocopherol. LDL glycation were estimated by sodium periodate assay. Based on this study results, ¦Á-tocopherol, ascorbic acid and lycopene decrease LDL glycation in a dose dependent manner. The electrophoretic mobility of glycated LDL decreased in presence these nutrients. These effects may be due to antioxidant properties of these nutrients and may have a role in ameliorating atherosclerotic risk of patients with diabetes mellitus.
%K Low density lipoprotein (LDL)
%K Glycation
%K ¦Á-Tocopherol
%K Ascorbic acid
%K Lycopene
%U http://www.ijpr-online.com/docs/20074/IJPR448.pdf