%0 Journal Article %T Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran %A GR Habibi %A AR Imani %A MR Gholami %A MH Hablolvarid %J Iranian Journal of Parasitology %D 2012 %I Tehran University of Medical Sciences %X Background: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. Methods: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathologi-cal observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally. Results: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three pas-sages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections. Conclusion: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes. %K Toxoplasma gondii %K Sheep abortion %K Fetus %K PCR %K Bioassay %K Genotyping %U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/22126.pdf&manuscript_id=22126