%0 Journal Article
%T Preparation and biodistribution study of 67Ga-gallium calcitonin
%A Amir Reza Jalilian
%A Mohammad Amin Hosseini
%A Fatemeh Bolourinovin
%J Iranian Journal of Nuclear Medicine
%D 2011
%I 
%X Introduction: In order to develop a radiolabeled calcitonin (CT) derivative for receptor imaging studies, CT was successively labeled with 67Ga-gallium chloride.Methods: The best results of the conjugation were obtained by the addition of 0.5 ml of a CT nasal pharmaceutical solution (1100 IU) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25ˇăC with continuous mild stirring for 30 min.Results: After solid phase purification of the radiolabeled hormone, instant thin layer chromatography (ITLC) showed radiochemical purity of higher than 95 % at optimized conditions (specific activity =67-134 KBq/IU, labeling efficiency 70%). 67Ga-DTPA-CT mainly accumulates in the liver.Conclusion: Preliminary in vivo studies (ID/g%) in male wild-type rats showed significant liver uptake of the tracer after 24 hours. 67Ga-DTPA-CT can be a suitable probe for biodistribution study of CT receptors in various physiological as well as neoplastic lesions with over-expressed calcitonin receptors
%K Calcitonin
%K Radiolabeling
%K Biodistribution
%K Ga-67
%U http://irjnm.tums.ac.ir/index.php/irjnm/article/view/708/665