%0 Journal Article
%T Dye Labelled Monoclonal Antibody Assay for Detection of Toxic Shock Syndrome Toxin -1 from Staphylococcus Aureus
%A V Javid Khojasteh
%A HA Foster
%J Iranian Journal of Microbiology
%D 2011
%I Tehran University of Medical Sciences
%X Objective: The aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (DLMAA), using non-radioactive organic synthetic dyes for identification of Toxic Shock Syndrome Toxin-1 (TSST-1) producing strains of Staphylococcus aureus.Materials and Methods: The assay protocol required only two simple steps; addition of TSST-1 antigen to a nitrocellulose membrane and then adding a colloidal dye labelled antibody (D/A) suspension detection reagent.Results: The sensitivity and specificity of the assay was determined relative to positive and negative strains compared to an ELISA assay. Overall 100% agreement was found between both assays. The sensitivity for detection of TSST-1 was 30 ng.Conclusion: The DLMAA did not require handling and disposal of radioactive materials. It is a rapid qualitative technique for detection of TSST-1 toxin at room temperature within a short time.
%K Monoclonal Antibody
%K Staphylococcus Aureus
%K TSST-1
%K Colloidal Dye
%K ELISA
%U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20428.pdf&manuscript_id=20428