%0 Journal Article
%T Citotoxicity of different amounts of sodium hypochlorite on human cultured osteoblasts
%A Tatiana Kelly da Silva Fidalgo
%A Roberta Barcelos
%A D¨¦bora Barreiros Petr¨®polis
%A Bruno Rocha Azevedo
%J RGO : Revista Ga¨²cha de Odontologia
%D 2009
%I Mundi Brasil Gr¨¢fica e Editora Ltda.
%X Objective: To evaluate the cytotoxic effect of different amounts of sodium hypochlorite, on a culture of human osteoblastos (HOB) cells.Method: Cultures of human osteoblasts (HOB) in Dulbecco¡¯s modified Eagle¡¯s medium (DMEM) supplemented with 10% of bovine fetal serum were incubated in sodium hypochlorite (concentrations of 0.5; 1.0; 2.5 and 5.25%) for thirty seconds. The control group was represented by cells incubated in phosphate buffered saline (PBS). Cell viability was assessed by means of 0.4% trypan blue dye exclusion test, in triplicate. During the incubation period, images were recorded through an inverted optic microscope to evaluate the cellular morphology. Results: In the control group 98.7% of viable cells were verified, without morphology alterations, while no viable cells were observed in the experimental groups. The kinetics of cytotoxity was concentration-dependent. Conclusion: It was concluded that there was a cytotoxic effect on cultures of human osteoblasts incubated for thirty seconds in sodium hypochlorite in all concentrations (0.5; 1.0; 2.5 and 5.25%)
%K sodium hypochlorite
%K root canal irrigants
%K osteoblas
%K toxicity
%K hipoclorito de s¨®dio
%K irrigantes do canal radicular
%K osteoblastos
%K toxicidade
%U http://www.revistargo.com.br/include/getdoc.php?id=3953&article=853&mode=pdf