%0 Journal Article
%T Induction of microRNA-214-5p in human and rodent liver fibrosis
%A Masashi Iizuka
%A Tomohiro Ogawa
%A Masaru Enomoto
%A Hiroyuki Motoyama
%A Katsutoshi Yoshizato
%A Kazuo Ikeda
%A Norifumi Kawada
%J Fibrogenesis & Tissue Repair
%D 2012
%I BioMed Central
%R 10.1186/1755-1536-5-12
%X The expression of miR-214-5p and genes that are involved in liver fibrosis were analyzed in hepatitis C virus-infected human livers, rodent fibrotic livers, a human stellate cell line (LX-2), and the cells from intact mouse livers using real-time PCR. The effect of miR-214-5p overexpression in LX-2 cells on cell function was investigated. Twist-1 expression in the liver tissues of mouse models and primary-cultured stellate cells was also analyzed.miR-214-5p was upregulated in human and mouse livers in a fibrosis progression¨Cdependent manner. miR-214-5p expression increased during the culture-dependent activation of mouse primary stellate cells and was significantly higher in stellate cells than in hepatocytes. The overexpression of miR-214-5p in LX-2 cells increased the expression of fibrosis-related genes, such as matrix metalloproteinase (MMP)-2, MMP-9, ¦Á-smooth muscle actin, and transforming growth factor (TGF)-¦Â1. TGF-¦Â stimulation induced miR-214-5p in LX-2 cells. Twist-1 was increased in fibrotic mouse livers and induced during mouse stellate cell activation.miR-214-5p may play crucial roles in the activation of stellate cells and the progression of liver fibrosis. Twist-1 may regulate miR-214-5p expression in the liver, particularly in stellate cells.Liver fibrosis is a consequence of chronic liver trauma caused by hepatitis B or hepatitis C virus (HCV) infection, alcohol abuse, or steatohepatitis, which ultimately leads to liver cirrhosis, liver failure, and hepatocellular carcinoma [1]. Liver fibrosis is characterized by an abnormal accumulation of extracellular matrix (ECM) components, including types I and III collagen, laminin, and proteoglycans, in the liver parenchyma [2,3]. Transforming growth factor (TGF)-¦Â, which is produced and released by activated macrophages and platelets at the site of local inflammation, is considered to play a primary role in the fibrotic process [3]. Hepatic stellate cells - which are localized in Disse¡¯s space, store vitami
%K Collagen
%K Hepatocyte
%K Non-coding RNA
%K Stellate cell
%K Transforming growth factor-¦Â
%U http://www.fibrogenesis.com/content/5/1/12