%0 Journal Article
%T Serologic host response to Helicobacter pylori and Campylobacter jejuni in socially housed Rhesus macaques (Macaca mulatta)
%A Sabine Kienesberger
%A Guillermo I Perez-Perez
%A Juan L Rivera-Correa
%A Rafael Tosado-Acevedo
%A Huilin Li
%A Andre Dubois
%A Janis A Gonzalez-Martinez
%A Maria Dominguez-Bello
%A Martin J Blaser
%J Gut Pathogens
%D 2012
%I BioMed Central
%R 10.1186/1757-4749-4-9
%X Two cohorts of monkeys were analyzed. The first cohort consisted of 30 monkeys and was used to establish an enzyme-linked immunosorbent assay (ELISA) for H. pylori antibodies detection. To evaluate colonization of those macaques, stomach biopsies were collected and analyzed for the presence of H. pylori by histology and culture. C. jejuni ELISAs were established using human serum with known C. jejuni antibody status. Next, plasma samples of the 89 macaques (Cohort 2) were assayed for antibodies and then statistically analyzed.An H. pylori IgG ELISA, which was 100% specific and 93% sensitive, was established. In contrast, the IgA ELISA was only 82% specific and 61% sensitive. The CagA IgG assay was 100% sensitive and 61% of the macaques were positive. In cohort 2, 62% macaques were H. pylori sero-positive and 52% were CagA positive. The prevalence of H. pylori IgG and CagA IgG increased with monkey age as described for humans. Of the 89 macaques 52% showed IgG against C. jejuni but in contrast to H. pylori, the sero-prevalence was not associated with increasing age. However, there was a drop in the IgG (but not in IgA) mean values between infant and juvenile macaques, similar to trends described in humans.Rhesus macaques have widespread exposure to H. pylori and C. jejuni, reflecting their social conditions and implying that Rhesus macaques might provide a model to study effects of these two important human mucosal bacteria on a population.Helicobacter pylori are Gram-negative bacteria that colonize the gastric mucosa of humans across the world. However, H. pylori is disappearing from populations in developed countries [1,2]. In developing countries, up to 90% of the adult population carries the organism [3,4]. H. pylori is acquired early in life [5,6] and generally persists unless hosts are treated with antibiotics [1]. Gastric H. pylori colonization increases risk of peptic ulcer disease as well as adenocarcinoma of the distal stomach [7]. In addition to negative e
%K Helicobacter pylori
%K Campylobacter jejuni
%K Rhesus macaques
%K Antibodies
%K Sero-prevalence
%K CagA
%U http://www.gutpathogens.com/content/4/1/9