%0 Journal Article
%T Utilization of I百B每EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-百B Inhibitor Activity
%A Yu-Ling Lin
%A Yen-Shun Chen
%A Jui-Hung Hsieh
%A Ching-Min Lin
%A Hsin-Yi Wu
%A Chen-Si Lin
%A Rea-Min Chu
%A Kuang-Wen Liao
%A Yuan-Hsun Hsu
%J Biological Procedures Online
%D 2010
%I BioMed Central
%R 10.1007/s12575-010-9033-9
%X NF-百B is an inducible transcription factor involved in the regulation of gene expression for cytokine release, anti-apoptosis, adhesion molecule, and cell cycle regulation [1]. There are five members in the NF-百B family composed of p50/p105, p52/p100, c-Rel, Rel A, and Rel B [2]. These components all have a consensus amino acid domain that is Rel homology domain (RHD). RHD, a regulatory element for activity of NF-百B, is involved in NF-百B dimerization and I百B binding. Without intracellular stimulation, I百B associates with RHD of NF-百B to inhibit NF-百B migration into the nucleus and retain NF-百B in cytosol. As extracellular signals of NF-百B activation transduce into cells, I百B will be phosphorylated and results in degradation. The degradation of I百B can abolish the inhibitory effect on the activity of NF-百B. Therefore, the amount of intracellular I百B can be an indicator for the activity of NF-百B.The microbial metabolite is an important source of certain medical reagents. Particularly, many investigators indicated that microbial metabolites provide a source of abundant antioxidants. Previous investigations revealed that antioxidants have the ability to inhibit activity of NF-百B [3-5]. Antioxidants, such as 汕-catenin and bortezomib, have been reported to have a cytotoxic ability against tumor cells by inhibiting the activity of NF-百B [6,7]. Therefore, utilizing the principle of NF-百B activity regulation to develop a drug discovery system may help to rapidly find new drugs.In this study, we have focused on the establishment of a rapid and precise screening system with NF-百B inhibitory activity, and the metabolite products of microbes and the natural extract propolis were tested in this system. The plasmid pI百B每EGFP, which contained a chimeric gene that encodes a fusion protein, green fluorescent protein (EGFP) fused with I百B-汐 (I百B每EGFP), was used to establish the screening system. The pI百B每EGFP was transfected into cells and the transfected cells were treated with micro
%K Microbial metabolite
%K Antioxidant
%K I百B
%K EGFP
%K Hydroquinone
%K Propolis
%U http://www.biologicalproceduresonline.com/content/12/1/131