%0 Journal Article
%T Novel mouse mammary cell lines for in vivo bioluminescence imaging (BLI) of bone metastasis
%A Celeste M Bolin
%A Caleb Sutherland
%A Ken Tawara
%A Jim Moselhy
%A Cheryl L Jorcyk
%J Biological Procedures Online
%D 2012
%I BioMed Central
%R 10.1186/1480-9222-14-6
%X The 4 T1.2 luc3 and 66c14 luc2 cell lines were shown to have high levels of bioluminescence intensity in vitro and in vivo after orthotopic injection into mouse mammary fat pads. The 4 T1.2 luc3 cell line was found to closely model the sites of metastases seen in human patients including lung, liver, and bone. Specifically, 4 T1.2 luc3 cells demonstrated a high incidence of metastasis to spine, with an ex-vivo BLI intensity three orders of magnitude above the commercially available 4 T1 luc2 cells. 66c14 luc2 cells also demonstrated metastasis to spine, which was lower than that of 4 T1.2 luc3 cells but higher than 4 T1 luc2 cells, in addition to previously unreported metastases in the liver. High osteolytic activity of the 4 T1.2 luc3 cells in vivo in the bone microenvironment was also detected.The engineered 4 T1.2 luc3 and 66c14 luc2 cell lines described in this study are valuable tools for studying the cellular events moderating the metastasis of breast tumor cells to bone.
%K Breast cancer
%K Mammary cancer
%K Bone metastasis
%K in vivo imaging
%K 4 T1 cells
%K 4 T1.2 cells
%K Osteolysis
%K Syngeneic Balb/c model
%U http://www.biologicalproceduresonline.com/content/14/1/6/abstract