%0 Journal Article
%T Decreased D2-40 and increased p16INK4A immunoreactivities correlate with higher grade of cervical intraepithelial neoplasia
%A Hongxiu Han
%A Yan Yang
%A Zhouping Lu
%A Qizhi He
%A Zhenhua Lin
%J Diagnostic Pathology
%D 2011
%I BioMed Central
%R 10.1186/1746-1596-6-59
%X In this study, the immunoreactive patterns of D2-40, compared with p16INK4A, which is currently considered as the useful marker for cervical cancers and their precancerous diseases, were examined in total 125 cervical specimens including 32 of CIN1, 37 of CIN2, 35 of CIN3, and 21 of normal cervical tissue. D2-40 and p16INK4A immunoreactivities were scored semiquantitatively according to the intensity and/or extent of the staining.Diffuse D2-40 expression with moderate-to-strong intensity was seen in all the normal cervical epithelia (21/21, 100%) and similar pattern of D2-40 immunoreactivity with weak-to-strong intensity was observed in CIN1 (31/32, 97.2%). However, negative and/or focal D2-40 expression was found in CIN2 (negative: 20/37, 54.1%; focal: 16/37, 43.2%) and CIN3 (negative: 22/35, 62.8%; focal: 12/35, 34.3%). On the other hand, diffuse immunostaining for p16INK4A was shown in 37.5% of CIN1, 64.9% of CIN2, and 80.0% of CIN3. However, the immunoreactive pattern of D2-40 was not associated with the p16INK4A immunoreactivity.Immunohistochemical analysis of D2-40 combined with p16INK4A may have a significant implication in clinical practice for better identifying the grade of cervical intraepithelial neoplasia, especially for distinguishing CIN1 from CIN2/3.Although the histological assessment of cervical biopsies is often considered as the "gold standard", evaluating the grade of cervical intraepithelial neoplasia (CIN) by conventional light microscopy, especially distinguishing CNI1 from CIN2/3, often presents a diagnostic issue in surgical pathology [1]. There has been much recent attention regarding use of p16 immunoreactivity for the detection of high grade cervical squamous lesions, however, assessment of its clinical applications is seriously hampered by lack of standardized methodology [2]. Novel markers are needed to apply on histological specimens to identify the grade of cervical intraepithelial neoplasia when the lesion is morphologically difficu
%K D2-40
%K cervical intraepithelial neoplasia
%K immunohistochemistry
%K p16INK4A
%U http://www.diagnosticpathology.org/content/6/1/59