%0 Journal Article
%T Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-¦Â1
%A Andrea Star£¿¨ªchov¨¢
%A Luk¨¢£¿ Kubala
%A Eva Lincov¨¢
%A Zuzana Pernicov¨¢
%A Alois Kozub¨ªk
%A Karel Sou£¿ek
%J Biological Procedures Online
%D 2009
%I BioMed Central
%R 10.1007/s12575-009-9017-9
%X The phenomenon of plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of serious pathological states. Epithelial¨Cmesenchymal transition (EMT) is a crucial process in embryogenesis, but it also occurs during progression of tumors derived from epithelial cells (for review, see [1]). The transforming growth factor-¦Â1 (TGF-¦Â1) is an important growth factor inducing remodeling of epithelial cells. TGF-¦Â1 induces a complex change of the gene expression profile, which leads to the induction of cell cycle arrest, increased cell migration, and spreading [2-4]. In general, determination of the quality and quantity of remodeling of epithelial cells is a complex issue. It usually includes quantification of expression of epithelial and mesenchymal markers (E-cadherin, N-cadherin, and vimentin), visualization of cytoskeletal rebuilding (F-actin), migration, and invasive assay (wound healing and migration through Matrigel matrix; [5]). Conventionally, most of the approaches mentioned are based on a time-consuming end-point analysis of the state of whole cell populations combined with advanced techniques of analysis of individual cells with the use of flow cytometry or digital microscopic techniques and image analysis. However, neither the episodic nor the spatial resolution of these techniques is capable of registering very small and fast changes in cellular morphology. Currently, label-free and noninvasive methods based on electronic cell sensor arrays were suggested for the monitoring of cell physiology, particularly adhesion, spreading, and transient changes in cell morphology [6-9]. To widely accept this methodological approach and to correctly and precisely interpret data for these measurements is crucial to obtain precise correlation with cell morphology and overall phenotype using a relevant reference method. However, well-described models applying this methodological approach with diff
%K real-time cell analysis
%K cell plasticity
%K epithelial¨Cmesenchymal transition
%K transforming growth factor-¦Â1
%K F-actin
%K cytoskeleton remodeling
%U http://www.biologicalproceduresonline.com/content/11/1/316