%0 Journal Article
%T Determination of the role of calcium on instability of neurotoxic metabolite of ecstasy by HPTLC-mass
%A Bardia Jamali
%A Yalda Hosseinzadeh Ardakani
%A Mohammad-Reza Rouini
%A Alireza Foroumadi
%A Salimeh Amidi
%A Vahid Hossein Aghdam
%A Farzad Kobarfard
%J DARU Journal of Pharmaceutical Sciences
%D 2013
%I BioMed Central
%R 10.1186/2008-2231-21-9
%X The stability of HHMA was studied in the perfusion medium and water at 100 and 10 ng/mL concentrations. Moreover, the effect of temperature (0--25 [degree sign]C), pH (3--10), calcium chloride (0--150 g/L) and ethylenediaminetetraacetic acid (EDTA) on the stability of HHMA was also examined.Our result suggested that the free form of HHMA could be degraded in the perfusion medium. The rate of this degradation has direct proportion to temperature (at 25[degree sign]C = 0.037 min-1 and at 0[degree sign]C = 0.002 min-1). Calcium chloride and sodium bicarbonate are two responsible components in this instability. Moreover, the alkaline pHs and increasing the shaking time can accelerate this effect. Although, while degradation was prevented at pH=3, EDTA could only reduce this rate about 30%.Calcium cation can act as an accelerator of HHMA degradation. Therefore, the perfusion medium should not contain Ca2+ and the pH of medium is better to be adjusted at acidic range. Since, the internal cellular source of calcium is endoplasmic reticulum system, it can be assumed that, this cation may change HHMA and dopamine to reactive compounds that can bind covalently to the cysteinyl group of biological compounds and damage cellular components.
%U http://www.darujps.com/content/21/1/9/abstract