%0 Journal Article
%T Anticancer activity of Pupalia lappacea on chronic myeloid leukemia K562 cells
%A Alvala Ravi
%A Mallika Alvala
%A Venkatesh Sama
%A Arunasree M Kalle
%A Vamshi K Irlapati
%A B Reddy
%J DARU Journal of Pharmaceutical Sciences
%D 2012
%I BioMed Central
%R 10.1186/2008-2231-20-86
%X Antiproliferative activity of EAPL was determined by MTT assay using carvacrol as a positive control. Induction of apoptosis was studied by annexin V, mitochondrial membrane potential, caspase activation and cell cycle analysis using flow cytometer and modulation in protein levels of p53, PCNA, Bax and Bcl2 ratio, cytochrome c and cleavage of PARP were studied by Western blot analysis. The standardization of the extract was performed through reverse phase-HPLC using Rutin as biomarker.The results showed dose dependent decrease in growth of K562 cells with an IC50 of 40£¿¡À£¿0.01£¿¦Ìg/ml by EAPL. Induction of apoptosis by EAPL was dose dependent with the activation of p53, inhibition of PCNA, decrease in Bcl2/Bax ratio, decrease in the mitochondrial membrane potential resulting in release of cytochrome c, activation of multicaspase and cleavage of PARP. Further HPLC standardization of EAPL showed presence 0.024% of Rutin.Present study significantly demonstrates anticancer activity of EAPL on Chronic Myeloid Leukemia (K562) cells which can lead to potential therapeutic agent in treating cancer. Rutin, a known anti cancer compound is being reported and quantified for the first time from EAPL.Plant derived natural products gaining importance to cure various disease conditions. Side effects of several allopathic drugs and development of resistance to currently used drugs have led to increased emphasis on the use of plant materials as a source of medicines for a wide variety of human ailments. Incidentally plants and herbs are persistently being studied for the identification of novel therapeutic agents. Among the 2, 50,000 higher plant species on earth, more than 80,000 plants have medicinal values. India is one of the biodiversity centers with the presence of over 45,000 different plant species. Of these, about ~20,000 plants have good medicinal value. However, only ~7500 species are used for their medicinal values by traditional communities. It is well established that plan
%K Pupalia lappacea
%K Anticancer activity
%K Chronic myeloid leukemia (K562) cells
%K Cytochrome c
%K p53
%K Multicaspase
%K PCNA
%U http://www.darujps.com/content/20/1/86