%0 Journal Article
%T Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum)
%A Chioma A Anosike
%A Onyechi Obidoa
%A Lawrence US Ezeanyika
%J DARU Journal of Pharmaceutical Sciences
%D 2012
%I BioMed Central
%R 10.1186/2008-2231-20-76
%X Twenty five (25) adult Wistar rats of either sex (120 g ¨C 200 g) divided into five groups of five rats each were used for each of the animal models. Groups 2, 3 and 4 were administered varied doses of the extract (100, 200 and 400 mg/kg), while groups 1 (vehicle control) and 5 (treatment control) received normal saline and indomethacin (50 mg/kg) respectively. Vascular permeability was induced by the intra-peritoneal injection of 1 ml of acetic acid and monitored using 0.5 ml intravenous injection of 1% Evans blue solution. Leukocyte mobilization was induced by the intra-peritoneal injection of 0.5 ml of 3% agar suspension in normal saline. Heat and hypotonicity induced heamolysis of HRBC membrane was used to assess membrane stabilization.The methanol extracts of garden egg significantly and dose dependently reduced (p¡Ü0.05) the acetic acid induced vascular permeability and agar induced leukocyte mobilization in rats. The percentage inhibitions of induced vascular permeability were 21 ¡À 3.39, 25 ¡À1.92 and 60 ¡À 3.81 for the 100, 200 and 400 mg/kg of the extract while the inhibitions of the agar induced leucocyte migration were 23 ¡À 2.17, 26 ¡À 1.58 and 32 ¡À 1.58 for the 100, 200 and 400 mg/kg of the extract respectively. The extract also, at doses of 100, 200, 400, 600 and 800 ¦Ìg/ml significantly inhibited heat induced lysis of the human red cell membrane with values of 66.46 ¡À 2.89, 65.14 ¡À 4.58, 46.53 ¡À 2.52, 61.88 ¡À 4.51and 86.67 ¡À 3.06 respectively.These results show that methanol extract of Solanum aethiopicum has anti-inflammatory properties and can reduce inflammatory injury and tissue damage.Inflammation is a complex biological response of vascular tissues to harmful stimuli. It is also a protective attempt by the organism to remove the injurious stimuli and initiate the healing process [1]. At the onset of an inflammation, the cells undergo activation and release inflammatory mediators. These mediators include histamine, serotonin, slow reacting substances of
%K Inflammation
%K Leucocyte migration
%K Vascular permeability
%K Human red cell membrane
%U http://www.darujps.com/content/20/1/76