%0 Journal Article
%T Inhibition of HIV and HSV infection by vaginal lactobacilli in vitro and in vivo
%A Rezvan Zabihollahi
%A Elahe Motevaseli
%A Seyed Sadat
%A Ali Azizi-Saraji
%A Sogol Asaadi-Dalaie
%A Mohammad Modarressi
%J DARU Journal of Pharmaceutical Sciences
%D 2012
%I BioMed Central
%R 10.1186/2008-2231-20-53
%X The in vitro anti-viral effect of vaginal and non-vaginal lactobacillus was evaluated using single cycle HIV-1 replication and HSV-2 plaque reduction assays. The XTT proliferation assay was used to monitor the cellular toxicity. The in vivo anti-HSV-1 activity was evaluated in BALB/c mouse model by monitoring skin lesion and immune response development.DMEM culture supernatant of L. Gasseri and L. fermentum (PH 7.3) did not show toxic effect but inhibited 50% of HIV replication at 12 and 31% concentrations, respectively. Co-culture of L. gasseri (1000 CFU/ target cell) showed mild cytotoxicity but inhibited 68% of HIV replication. The supernatant of L. crispatus inhibited 50% of HSV replication at 4% and also co-culture of L. gasseri, L. rhamnosus and L. crispatus revokes almost all of the HSV multiplication. Culture supernatants of L. gasseri and L. crispatus had significant virucidal effect against the HIV and HSV and inhibited HSV infection in a stage before viral entry to the target cells. Alive L. gasseri cells showed high potential for inhibiting HSV-1 infection in vivo condition. Current data indicates that lactobacilli supernatant encompasses components with neutralizing activity against HIV and HSV and it would be a determinant factor for viral diseases transmission and promising lead for anti-viral probiotic design.The cervico-vaginal mucosa is the portal of entry for sexually transmitted pathogenic microorganisms. In child-bearing age healthy females, the vaginal protective mucosa is populated with microflora that mostly includes lactobacilli. Vaginal health is positively associated with dominance of lactobacilli over pathogenic anaerobes [1]. Bacterial vaginosis (BV) is a situation with an imbalance of the vaginal microbial flora and is not caused by specific pathogenic microorganisms [2]. Reduction, absence or lack of antimicrobial properties (production of acid and H2O2) of lactobacilli and their replacement by anaerobic microbes such as Gardnerella va
%K HIV
%K HSV
%K Sexually transmitted disease
%K Lactobacillus
%U http://www.darujps.com/content/20/1/53