%0 Journal Article
%T A DNA microarray survey of gene expression in normal human tissues
%A Radha Shyamsundar
%A Young H Kim
%A John P Higgins
%A Kelli Montgomery
%A Michelle Jorden
%A Anand Sethuraman
%A Matt van de Rijn
%A David Botstein
%A Patrick O Brown
%A Jonathan R Pollack
%J Genome Biology
%D 2005
%I BioMed Central
%R 10.1186/gb-2005-6-3-r22
%X Here we describe a systematic survey of gene expression in 115 human tissue samples representing 35 different tissue types, using cDNA microarrays representing approximately 26,000 different human genes. Unsupervised hierarchical cluster analysis of the gene-expression patterns in these tissues identified clusters of genes with related biological functions and grouped the tissue specimens in a pattern that reflected their anatomic locations, cellular compositions or physiologic functions. In unsupervised and supervised analyses, tissue-specific patterns of gene expression were readily discernable. By comparative hybridization to normal genomic DNA, we were also able to estimate transcript abundances for expressed genes.Our dataset provides a baseline for comparison to diseased tissues, and will aid in the identification of tissue-specific functions. In addition, our analysis identifies potential molecular markers for detection of injury to specific organs and tissues, and provides a foundation for selection of potential targets for selective anticancer therapy.DNA microarrays [1,2] have been used to profile gene expression in cancer and other diseases. In cancer, for example, microarray profiling has been applied to classify tumors according to their sites of origin [3-5], to discover previously unrecognized subtypes of cancer [6-11], to predict clinical outcome [12-14] and to suggest targets for therapy [15,16]. However, the identification of improved markers for diagnosis and molecular targets for therapy will depend on knowledge not only of the genes expressed in the diseased tissues of interest, but also on detailed information about the expression of the corresponding genes across the gamut of normal human tissues.At present there is relatively little data on gene expression across the diversity of normal human tissues [17-20]. Here we report a DNA microarray-based survey of gene expression in a diverse collection of normal human tissues and also present an emp
%U http://genomebiology.com/2005/6/3/R22