%0 Journal Article
%T Increased expression of costimulatory markers CD134 and CD80 on interleukin-17 producing T cells in patients with systemic lupus erythematosus
%A Sebastian Dolff
%A Daniel Quandt
%A Benjamin Wilde
%A Thorsten Feldkamp
%A Fan Hua
%A Xin Cai
%A Christof Specker
%A Andreas Kribben
%A Cees GM Kallenberg
%A Oliver Witzke
%J Arthritis Research & Therapy
%D 2010
%I BioMed Central
%R 10.1186/ar3100
%X Thirty-four patients (3 male, 31 female, mean age 41 ¡À 15 years) fulfilling at least four of the American College of Rheumatology (ACR) revised criteria for the diagnosis of SLE and 24 healthy controls were enrolled. T-cells from the peripheral blood were analysed by fluorescence activated cell sorting (FACS) for their expression levels of CD80, CD134 and CCR6. In vitro stimulated CD3+IL17+ cells were also investigated for the expression of these costimulatory markers. Finally, renal biopsies from SLE patients were evaluated for the presence of CD134 expressing T-cells.Percentages of IL-17 expressing T-cells were significantly increased in patients with active disease as compared to healthy controls (1.46 ¡À 0.58% versus 0.93 ¡À 0.30%, P = 0.007). The percentage of IL-17 producing T-cells was correlated with disease activity as assessed by systemic lupus erythematosus disease activity index (SLEDAI) (r = 0.53, P = 0.003). In patients, most of the IL-17 producing T-cells were confined to the CCR6+ T-cell subset (80 ¡À 13%). Expression of CD80 and CD134 on the IL-17 producing T-cell subset was higher in SLE than in healthy controls (HC) (CD134: 71.78 ¡À 14.51% versus 51.45 ¡À 16.58%, P = 0.002; CD80: 25.5 ¡À 14.99% versus 14.99 ¡À 5.74%, P = 0.02). Also, patients with lupus nephritis expressed higher levels of CD134+ on CD3+IL-17+ cells as compared to HC (72.69 ¡À 11.54% versus 51.45 ¡À 16.58%, P = 0.006). Furthermore, renal biopsies of lupus nephritis patients showed infiltration of CD134+ T cells.Percentages of IL-17 expressing T-cells correlate with disease activity. Further, these cells show increased expression of costimulatory markers such as CD134 and CD80. The presence of CD134+ T-cells in renal biopsies of lupus nephritis patients suggest that these cells migrate to the kidney and might contribute to inflammatory processes through IL-17 secretion.Systemic lupus erythematosus (SLE) is a multiorgan autoimmune disease characterized by an imbalanced T cell homeostasis wit
%U http://arthritis-research.com/content/12/4/R150