%0 Journal Article
%T Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features
%A Raquel Celis
%A N¨²ria Planell
%A Jos¨¦ L Fern¨¢ndez-Sueiro
%A Raimon Sanmart¨ª
%A Julio Ram¨ªrez
%A Isidoro Gonz¨¢lez-¨¢lvaro
%A Jos¨¦ L Pablos
%A Juan D Ca£¿ete
%J Arthritis Research & Therapy
%D 2012
%I BioMed Central
%R 10.1186/ar3817
%X ST and paired SF samples were obtained from the inflamed knee of PsA patients. ST samples were immunostained with CD3 (T cell), CD20 (B cell), and MECA-79 (high endothelial vessels). Total ST mRNA was extracted, and the gene expression of 21 T-cell-derived and proinflammatory cytokines were measured with quantitative real-time PCR. SF concentrations of Th1, Th2, Th17, and proinflammatory cytokines were determined with the Quantibody Human Th17 Array. Clinical and biologic data were collected at inclusion and after a median of 27 months of follow-up.Twenty (43.5%) of 46 patients had LN. Only two genes showed differences (Wilcoxon test, P < 0.06) in ST between LN-positive and LN-negative patients: interleukin-23A (IL-23A) (P = 0.058) and transforming growth factor-beta (TGF-¦Â1) (P = 0.050). IL-23A expression was higher, and TGF-¦Â1 expression was lower in LN-positive patients. ST IL-15 mRNA showed a nonsignificant trend toward higher expression in LN-positive patients, and SF IL-15 protein levels were significantly higher in LN-positive patients (P = 0.002). In all PsA patients, IL-23A mRNA expression correlated with C-reactive protein (CRP) (r = 0.471; P = 0.001) and swollen-joint count (SJC) (r = 0.350; P = 0.018), whereas SF levels of IL-6 and CC chemokine-ligand 20 (CCL-20) correlated with CRP levels (r = 0.377; P = 0.014 and r = 0.501; P < 0.0001, respectively).These findings suggest differences in the cytokine profile of PsA patients with LN, with a higher expression of IL-23A and IL-15 and a lower expression of TGF-¦Â1. In the entire group of patients, IL-23 ST expression and CCL20 SF levels strongly correlated with markers of disease activity. This cytokine pattern was not accompanied by gross clinical or biologic differences between LN-positive and -negative patients. Taken together, these results suggest a role of the IL-17/IL-23 cytokine axis in synovial LN in PsA.Psoriatic arthritis (PsA) is a chronic, inflammatory, musculoskeletal disease associated with sk
%U http://arthritis-research.com/content/14/2/R93