%0 Journal Article
%T Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
%A Sebastian Worch
%A Kalladan Rajesh
%A Vokkaliga T Harshavardhan
%A Christof Pietsch
%A Viktor Korzun
%A Lissy Kuntze
%A Andreas B£¿rner
%A Ulrich Wobus
%A Marion S R£¿der
%A Nese Sreenivasulu
%J BMC Plant Biology
%D 2011
%I BioMed Central
%R 10.1186/1471-2229-11-1
%X The expression patterns of drought regulated genes were monitored during plant ontogeny, mapped and the location of these genes was incorporated into a comprehensive barley SNP linkage map. Haplotypes within a set of 17 starch biosynthesis/degradation genes were defined, and a particularly high level of haplotype variation was uncovered in the genes encoding sucrose synthase (types I and II) and starch synthase. The ability of a panel of 50 barley accessions to maintain grain starch content under terminal drought conditions was explored.The linkage/expression map is an informative resource in the context of characterizing the response of barley to drought stress. The high level of haplotype variation among starch biosynthesis/degradation genes in the progenitors of cultivated barley shows that domestication and breeding have greatly eroded their allelic diversity in current elite cultivars. Prospective association analysis based on core drought-regulated genes may simplify the process of identifying favourable alleles, and help to understand the genetic basis of the response to terminal drought.Drought is one of the most serious abiotic stress factors which occur throughout the development of the plant and, if sufficiently severe and/or prolonged, results in the modification of the plant's physiology and severely limit crop productivity. Plants have evolved a range of defence and escape mechanisms [1], and these are typically mediated by multiple rather than by single genes. In barley, QTL underlying drought tolerance has been mapped to almost every chromosome [2-6]. However, little information has been gathered to date regarding the genomic location of drought-regulated genes, either expressed throughout plant development or at late reproductive stages influencing seed yield and quality.Of all the genetic marker types available, single nucleotide polymorphisms (SNPs) are the most abundant, and thus offer the greatest level of genetic resolution. They are of potenti
%U http://www.biomedcentral.com/1471-2229/11/1