%0 Journal Article
%T Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed
%A Song-Lin Ruan
%A Hua-Sheng Ma
%A Shi-Heng Wang
%A Ya-Ping Fu
%A Ya Xin
%A Wen-Zhen Liu
%A Fang Wang
%A Jian-Xin Tong
%A Shu-Zhen Wang
%A Hui-Zhe Chen
%J BMC Plant Biology
%D 2011
%I BioMed Central
%R 10.1186/1471-2229-11-34
%X Phenotypic analysis of one protein that was upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated that OsCYP2 transgenic rice seedlings had better tolerance to salt stress than did wild-type seedlings. Interestingly, wild-type seedlings exhibited a marked reduction in maximal photochemical efficiency under salt stress, whereas no such change was observed for OsCYP2-transgenic seedlings. OsCYP2-transgenic seedlings had lower levels of lipid peroxidation products and higher activities of antioxidant enzymes than wild-type seedlings. Spatiotemporal expression analysis of OsCYP2 showed that it could be induced by salt stress in both Shanyou 10 and Liangyoupeijiu seedlings, but Shanyou 10 seedlings showed higher OsCYP2 expression levels. Moreover, circadian rhythm expression of OsCYP2 in Shanyou 10 seedlings occurred earlier than in Liangyoupeijiu seedlings. Treatment with PEG, heat, or ABA induced OsCYP2 expression in Shanyou 10 seedlings but inhibited its expression in Liangyoupeijiu seedlings. Cold stress inhibited OsCYP2 expression in Shanyou 10 and Liangyoupeijiu seedlings. In addition, OsCYP2 was strongly expressed in shoots but rarely in roots in two rice hybrid varieties.Together, these data suggest that OsCYP2 may act as a key regulator that controls ROS level by modulating activities of antioxidant enzymes at translation level. OsCYP2 expression is not only induced by salt stress, but also regulated by circadian rhythm. Moreover, OsCYP2 is also likely to act as a key component that is involved in signal pathways of other types of stresses-PEG, heat, cold, or ABA.Rice is a salt-sensitive cereal crop. High salinity may cause delayed seed germination, slow seedling growth, and reduced rate of seed set, leading to decreased rice yield. These disorders are generally due to the combined effects of ion imbalance, hyperosmotic stress, and oxidative damage. In the early period, rice can rapidly perceive a salt stress signal via plasma membrane recept
%U http://www.biomedcentral.com/1471-2229/11/34