%0 Journal Article
%T Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin
%A Nuria S¨˘nchez-Pons
%A Sami Irar
%A Nora Garc¨Şa-Muniz
%A Carlos M Vicient
%J BMC Plant Biology
%D 2011
%I BioMed Central
%R 10.1186/1471-2229-11-91
%X Comparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted. Under the conditions used in this study, camptothecin did not induce extensive degradation in the genomic DNA but induced the transcription of genes involved in DNA repair and repressed genes involved in cell division. Camptothecin also affected the accumulation of several proteins involved in the stress response and induced the activity of certain calcium-dependent nucleases. We also detected changes in the expression and accumulation of different genes and proteins involved in post-translational regulatory processes.This study identified several genes and proteins that participate in DNA damage responses in plants. Some of them may be involved in general responses to stress, but others are candidate genes for specific involvement in DNA repair. Our results open a number of new avenues for researching and improving plant resistance to DNA injury.Maintenance of genome stability is of critical importance for all organisms. Genomic DNA is continuously subject to many types of damage resulting from endogenous factors (production of reactive oxygen species, stalled replication forks, etc.) or the action of exogenous agents (radiation, naturally occurring radioisotopes, chemical mutagens such as heavy metals, etc.) [1]. Double-strand DNA breaks (DSBs) are one of the most serious forms of DNA damage, potentially causing chromosomal translocations and rearrangements [2]. In response to DSBs, cells initiate complex signalling pathways that activate DNA repair, cell-cycle arrest, and eventually cell death [3]. DSBs repair is mediated by two basic mechanisms: homologous recombination (HR) and non-homologous end joining (NHEJ) [4]. In HR, an intact copy of the damaged region (a sister chromatid, for example) acts as a template to repair the break. In NHEJ, DSBs are simply rejoined largely independently of the DNA sequence. Bacteria and yeast usually employ H
%U http://www.biomedcentral.com/1471-2229/11/91