%0 Journal Article
%T High-throughput sequencing of black pepper root transcriptome
%A Sheila MC Gordo
%A Daniel G Pinheiro
%A Edith CO Moreira
%A Simone M Rodrigues
%A Marli C Poltronieri
%A Oriel F de Lemos
%A Israel da Silva
%A Rommel TJ Ramos
%A Artur Silva
%A Horacio Schneider
%A Wilson A Silva
%A Iracilda Sampaio
%A Sylvain Darnet
%J BMC Plant Biology
%D 2012
%I BioMed Central
%R 10.1186/1471-2229-12-168
%X The root transcriptome of black pepper was sequenced by the NGS SOLiD platform and assembled using the multiple-k method. Blast2Go and orthoMCL methods were used to annotate 10338 unigenes. The 4472 predicted proteins showed about 52% homology with the Arabidopsis proteome. Two root proteomes identified 615 proteins, which seem to define the plant¡¯s root pattern. Simple-sequence repeats were identified that may be useful in studies of genetic diversity and may have applications in biotechnology and ecology.This dataset of 10338 unigenes is crucially important for the biotechnological breeding of black pepper and the ecogenomics of the Magnoliids, a major group of basal angiosperms.Black pepper (Piper nigrum) is one of the most popular and oldest spices in the world, with culinary uses and more recently as a food preservative [1]. Due its medicinal properties, it is used in traditional medicine for its antioxidant, anti-inflammatory and anticancer properties [2,3]. This species is the second most widely traded spice in the world, accounting for a value of 929 million US dollars from a production of 445900 tons in 2009 [4].P. nigrum is a perennial, climbing vine indigenous to the southwestern region of India. Its production is limited to rainy tropical regions such as those found in Asia and South America. Tropical climates favor the development of diseases, and crop losses due to pests and diseases are a major constraint in the production of black pepper [1]. Resistance against the two major pathogens, Phytophtora capsici and Fusarium solani f. sp. piperis, has not been achieved by classical breeding of the germplasm of cultivated black pepper and remains a major challenge that may be amenable to improvement using plant biotechnology [1]. Despite the agricultural and economic importance of black pepper, knowledge of its genetics is presently very limited [5].For non-model plants such as black pepper with little or no molecular information available, next-generation s
%U http://www.biomedcentral.com/1471-2229/12/168