%0 Journal Article
%T Transcriptome variation along bud development in grapevine (Vitis vinifera L.)
%A Jos¨¦ D¨ªaz-Riquelme
%A J¨¦r£¿me Grimplet
%A Jos¨¦ M Mart¨ªnez-Zapater
%A Mar¨ªa J Carmona
%J BMC Plant Biology
%D 2012
%I BioMed Central
%R 10.1186/1471-2229-12-181
%X Gene expression changes were analyzed along the bud annual cycle at eight different time points. Principal Components Analysis (PCA) revealed that the main factors explaining the global gene expression differences were the processes of bud dormancy and active growth as well as stress responses. Accordingly, non dormant buds showed an enrichment in functional categories typical of actively proliferating and growing cells together with the over abundance of transcripts belonging to stress response pathways. Differential expression analyses performed between consecutive time points indicated that major transcriptional changes were associated to para/endodormancy, endo/ecodormancy and ecodormancy/bud break transitions. Transcripts encoding key regulators of reproductive development were grouped in three major expression clusters corresponding to: (i) transcripts associated to flowering induction, (ii) transcripts associated to flower meristem specification and initiation and (iii) transcripts putatively involved in dormancy. Within this cluster, a MADS-box gene (VvFLC2) and other transcripts with similar expression patterns could participate in dormancy regulation.This work provides a global view of major transcriptional changes taking place along bud development in grapevine, highlighting those molecular and biological functions involved in the main events of bud development. As reported in other woody species, the results suggest that genes regulating flowering could also be involved in dormancy regulatory pathways in grapevine.Woody perennial plant species have specific morphological and physiological constraints when compared with annual plants, leading to different reproductive and somatic developmental strategies. Polycarpic woody plants develop terminal or axillary buds with embryonic shoots from which complete branches can develop after specific signals [1]. To elude unfavorable environmental conditions, these buds become dormant providing the possibility to res
%K Grapevine
%K Bud development
%K Dormancy
%K Flowering
%K Flower development
%K Transcriptomics
%U http://www.biomedcentral.com/1471-2229/12/181