%0 Journal Article
%T Effect of temperature on leukocyte activation during cardiopulmonary bypass (CPB) and postoperative organ damage
%A M Qing
%A M-C Seghaye
%A JF Vazquez-Jimenez
%A RG Grabitz
%A B Klosterhalfen
%A M Sigler
%A BJ Messmer
%A G von Bernuth
%J Critical Care
%D 1999
%I BioMed Central
%R 10.1186/cc331
%X 18 young pigs were randomly assigned to a T¡ã- group during CPB: normothermia (T¡ã 37¡ãC; n = 6), mild hypothermia (T¡ã 28¡ãC; n = 6) and deep hypothermia (T¡ã 20¡ãC; n = 6). Leukocyte count and plasma levels of tumor necrosis factor (TNF¦Á) were measured before, during and after CPB. At the end of the experimentation (6 h post-CPB), probes of heart, lungs, liver, kidney, and intestine were taken for histological examination.There was a significant fall of leukocyte count at induction of CPB, without any intergroup difference. During and at the end of CPB, leukocyte count was significantly higher in group 37¡ãC as compared with the other groups. At a later stage after CPB, group 20¡ãC showed significantly higher leukocyte count than group 28¡ãC and group 37¡ãC, respectively. The course of neutrophils was similar.TNF-¦Á was not released in group 28¡ãC neither during nor after CPB. By contrast, there was a significant production of TNF-¦Á in groups 37¡ãC and 20¡ãC, the circulating levels being significantly higher in group 37¡ãC. Histological examination showed that the most important tissue damage in terms of interstitial edema and leukostasis in heart, lung, liver, kidney, and small intestine was seen in group 37¡ãC followed by group 20¡ãC while the least important damage was present in group 28¡ãC.CPB-induced postoperative organ damage, probably related to leukocyte activation and TNF-¦Á production, is highest in pigs operated on in normothermia and lowest in those operated on in mild hypothermia.
%U http://ccforum.com/content/3/SA/P20