%0 Journal Article
%T Antimutagenic and free radical scavenger effects of leaf extracts from Accacia salicina
%A Jihed Boubaker
%A Hedi Mansour
%A Kamel Ghedira
%A Leila Chekir-Ghedira
%J Annals of Clinical Microbiology and Antimicrobials
%D 2011
%I BioMed Central
%R 10.1186/1476-0711-10-37
%X Antioxidant activity of A. salicina extracts was determined by the ability of each extract to protect against plasmid DNA strand scission induced by hydroxyl radicals. An assay for the ability of these extracts to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 and TA 104 strains was conducted. In addition, nonenzymatic methods were employed to evaluate anti-oxidative effects of tested extracts.These extracts from leaf parts of A. salicina showed no mutagenicity either with or without the metabolic enzyme preparation (S9). The highest protections against methylmethanesulfonate induced mutagenicity were observed with all extracts and especially chloroform extract. This extract exhibited the highest inhibitiory level of the Ames response induced by the indirect mutagen 2- aminoanthracene. All extracts exhibited the highest ability to protect plasmid DNA against hydroxyl radicals induced DNA damages. The ethyl acetate (EA) and chloroform (Chl) extracts showed with high TEAC values radical of 0.95 and 0.81 mM respectively, against the ABTS.+.The present study revealed the antimutagenic and antioxidant potenty of plant extract from Accacia salicina leaves.Exposure to genotoxic chemicals present in food, in the environment, and used in medical treatment can alter the genetic material permanently, and thus may lead cancer [1]. On the other hand, oxidative stress, caused by reactive oxygen species (ROS), is known to cause the oxidation of biomolecules, leading to cellular damage. The tissue injury caused by ROS may include DNA protein and lipids damage [2,3]. Antigenotoxic plant can counter or prevent the adverse effect caused by DNA-damaging chemicals [4]. Drugs obtained from plants have been investigated for the possible presence of mutagenic and/or carcinogenic substances, following the criteria and norms established for synthetic medicines. Fortunately, numerous defense systems protect the cellular macromolecules against oxidation. DNA repa
%U http://www.ann-clinmicrob.com/content/10/1/37