%0 Journal Article
%T Functional phosphoproteomic mass spectrometry-based approaches
%A Elena L¨®pez
%A Xiangdong Wang
%A Luis Madero
%A Juan L¨®pez-Pascual
%A Martin Latterich
%J Clinical and Translational Medicine
%D 2012
%I Springer
%R 10.1186/2001-1326-1-20
%X The study of proteomes and phosphoproteomes as linked systems, rather than research studies of individual proteins, are necessary to understand the functions of phosphorylated and un-phosphorylated proteins under spatial and temporal conditions. Phosphoproteome studies also facilitate drug target protein identification which may be clinically useful in the near future.Here, we provide an overview of general principles of signaling pathways versus phosphorylation. Likewise, we detail chemical phosphoproteomic tools, including pros and cons with examples where these methods have been applied. In addition, basic clues of electrospray ionization and collision induced dissociation fragmentation are detailed in a simple manner for successful phosphoproteomic clinical studies.Proteomics technologies are of great use for developing reference maps of targets of kinase inhibitors. This implies a difficult but promising issue, as it concerns the determination of the network-level response to different inhibitor treatments. The challenges and cautions associated with each method will be described in a simple manner.With advances of techniques available, the next decade should see a rapid increase in our understanding of signaling network regulation. This, at the same time, implies more efficient therapeutic strategies.Comparative proteomics can distinguish small but important changes in protein modifications in their structure, thus facilitating drug target protein identification.In addition, phosphoproteomic approaches can be exploited to monitor changes in phosphorylation events in order to characterize drug actions on cell signaling pathways and/or signaling cascades. Likewise, functional proteomic and phosphoproteomic approaches can be used to: (i) improve the knowledge or the clarification of the mechanism of a drug action for a specific disease, (ii) achieve relevant protein-identification of disease related to a specific signaling network and (iii) reach the important st
%K Phosphorylation
%K Signaling pathways
%K Phosphoproteomics
%K Mass spectrometry
%U http://www.clintransmed.com/content/1/1/20