%0 Journal Article
%T Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery
%A Robert Kruse
%A Birgitta Ess¨¦n-Gustavsson
%A Caroline Fossum
%A Marianne Jensen-Waern
%J Acta Veterinaria Scandinavica
%D 2008
%I BioMed Central
%R 10.1186/1751-0147-50-32
%X Ten conventional pigs (~23 kg) were orally inoculated with Brachyspira hyodysenteriae B204T. Eight animals developed muco-haemorrhagic diarrhoea with impaired general body condition. Blood was sampled before inoculation and repeatedly during acute dysentery and recovery periods and cytokine levels of IL-1¦Â, IL-6, Il-10, TNF-¦Á and IFN-¦Ã were measured by ELISA.IL-1¦Â was increased at the beginning of the dysentery period and coincided with the appearance of Serum amyloid A and clinical signs of disease. TNF-¦Á increased in all animals after inoculation, with a peak during dysentery, and IL-6 was found in 3 animals during dysentery and in the 2 animals that did not develop clinical signs of disease. IL-10 was found in all sick animals during the recovery period. IFN-¦Ã was not detected on any occasion.B. hyodysenteriae inoculation induced production of systemic levels of IL-1¦Â during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery.Swine dysentery is an important disease caused by the spirochete Brachyspira hyodysenteriae [1]. This infection is confined to the large intestine and results in muco-haemorrhagic diarrhoea, deterioration of general condition and a high mortality if untreated [2]. We have previously reported on the increase of numbers of neutrophils, monocytes and CD8¦Á+ lymphocytes during dysentery and the increase in ¦Ã¦Ä T cells and B. hyodysenteriae-specific antibodies during the recovery period [3,4]. The knowledge of the cytokine response during swine dysentery is still limited and most of the information available comes from in vitro studies [5-7]. However, considering the complexity of a natural infection, in which a multitude of factors are involved, in vivo findings are imperative for understanding various clinical responses to an infection.Locally produced cytokines may reach concentrations that are systemically detectable during infections. Increased amounts of pro-inflammatory cytokines generally have a negativ
%U http://www.actavetscand.com/content/50/1/32