%0 Journal Article
%T Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevyliteˋ antibodies. comparison with the free light chain oligoclonal pattern
%A David Zeman
%A Pavel Hrad赤lek
%A Zden豆k ˋvagera
%A Eva Mojˋ赤ˋkov芍
%A Ivana Woznicov芍
%A Olga Zapletalov芍
%J Fluids and Barriers of the CNS
%D 2012
%I BioMed Central
%R 10.1186/2045-8118-9-5
%X We have used Hevyliteˋ antibodies and developed a technique for detection of oligoclonal IgG百 and IgG竹 bands by means of isoelectric focusing followed by immunoblotting. The same technique was used for oligoclonal free 百 and free 竹 detection. Among several techniques tested, affinity immunoblotting appears to be the most sensitive; it can detect less than 1 ng of IgG百 or IgG竹 paraprotein. We compared oligoclonal IgG profiles with those of oligoclonal IgG百 and IgG竹. There was good agreement concerning the presence or absence of intrathecal synthesis. We observed the ratios between oligoclonal IgG百 and IgG竹 bands, and they did not always match the ratios between free 百 and free 竹 bands. We were also able to detect antigen-specific CSF-restricted oligoclonal IgG百 and IgG竹 bands in neuroborreliosis. It remains to be determined subsequently by a clinically-oriented prospective study, whether predominant IgG百/IgG竹 or free 百/free 竹 can be observed more frequently in particular diseases with oligoclonal IgG synthesis.Very sensitive detection of oligoclonal IgG百 and IgG竹 bands in cerebrospinal fluid with Hevylite antibodies is feasible; detection of antigen-specific IgG百 or IgG竹 is possible as well. In particular situations, e.g. when difficulties arise in distinguishing between oligoclonal and monoclonal pattern, the test may be of considerable clinical value.Oligoclonal IgG bands (OCBs) in cerebrospinal fluid (CSF) that are absent in serum indicate intrathecal IgG synthesis and are a sensitive marker of CNS inflammatory diseases, in particular multiple sclerosis (MS). Isoelectric focusing (IEF) and specific IgG detection by means of immunofixation (IF) or immunoblotting (IB) is the recommended procedure for OCBs detection [1-3]. It may be of interest to determine whether these bands are predominantly IgG百 or IgG竹. Previous reports used either quantitative tests [4-7] or immunofixation with kappa and lambda antisera [8-10] to demonstrate altered kappa/lambda or oligoclonal
%K Cerebrospinal fluid
%K Oligoclonal bands
%K IgG kappa
%K IgG lambda
%K Free light chains
%K Hevyliteˋ
%U http://www.fluidsbarrierscns.com/content/9/1/5