%0 Journal Article
%T Optimization of technology for dietary fiber extraction from Maixiansan by response surface methodology
%A Hui-qing Lv
%A Changfeng Hu
%A Hai-ping Zhong
%A Hong-bin Zheng
%A Chengping Wen
%J Chinese Medicine
%D 2012
%I BioMed Central
%R 10.1186/1749-8546-7-28
%X A Box每Behnken design was employed to optimize the extraction parameters, including 汐-amylase concentration (X1: 0.3 每 0.5%), enzymolysis time (X2: 30 每 60 min) and NaOH content (X3: 1.0 每 5.0%), of dietary fiber from Maixiansan using an enzyme每alkali extraction technique.The optimal technological conditions were as follows: 汐-amylase concentration: 0.4%; enzymolysis time: 45 min; NaOH content: 4.0%. Under these conditions, the extraction yield reached 57.14%, which was well consistent with the predicted models with a coefficient of determination (R2) of 0.9818. An evaluation of the anti-inflammatory activity indicated that Maixiansan was able to significantly inhibit dextran sodium sulfate-induced ulcerative colitis in rats by increasing the concentration of short-chain fatty acids (acetate, propionate and butyrate), among which the butyrate content was significantly higher in the Maixiansan group than in the other groups.Our experiments showed that response surface methodology can optimize the extraction of dietary fiber from Maixiansan. Maixiansan could be explored as an anti-ulcerative colitis agent.Inflammatory bowel disease includes Crohn＊s disease and ulcerative colitis (UC). UC is a chronic inflammatory disorder of the colon characterized by alternating periods of flare-ups and quiescent disease [1]. Dietary fiber is an integral constituent of normal human nutrition and may contribute to a healthy colonic environment [2]. The protective effects of a fiber-rich diet have been attributed to short-chain fatty acids (SCFAs), including acetate, propionate and butyrate [3]. SCFAs are the major end products of fiber fermentation in the gastrointestinal tract [4-6]. Our previous study revealed that germinated barley foodstuff (GBF) increased the numbers of eubacteria and bifidobacteria in experimental rats. Maixiansan, composed of GBF, tuckahoe and Chinese yam, can effectively prevent bloody diarrhea and mucosal damage, and exerts significant effects on UC [7].The co
%U http://www.cmjournal.org/content/7/1/28