%0 Journal Article
%T Identification of nine sequence types of the 16S rRNA genes of Campylobacter jejuni subsp. jejuni isolated from broilers
%A Ingrid Hansson
%A Marianne Persson
%A Linda Svensson
%A Eva Engvall
%A Karl-Erik Johansson
%J Acta Veterinaria Scandinavica
%D 2008
%I BioMed Central
%R 10.1186/1751-0147-50-10
%X The 16S rRNA genes of 45 strains of C. jejuni and two C. coli strains isolated from broilers were sequenced and compared with 16S rRNA sequences retrieved from the Ribosomal Database Project or GenBank. The strains were also genotyped by PFGE after digestion with SmaI.Sequence analyses of the 16S rRNA genes revealed nine sequence types of the Campylobacter strains and the similarities between the different sequence types were in the range 99.6¨C99.9%. The number of nucleotide substitutions varied between one and six among the nine 16S rRNA sequence types. One of the nine 16S rRNA sequence profiles was common to 12 of the strains from our study and two of these were identified as Campylobacter coli by PCR/REA. The other 10 strains were identified as Campylobacter jejuni. Five of the nine sequence types were also found among the Campylobacter sequences deposited in GenBank. The three 16S rRNA genes in the analysed strains were identical within each individual strain for all 47 strains.C. jejuni and C. coli seem to lack polymorphisms in their 16S rRNA gene, but phylogenetic analysis based on 16S rRNA sequences was not always sufficient for differentiation between C. jejuni and C. coli. The strains were grouped in two major clusters according to 16S rRNA, one cluster with only C. jejuni and the other with both C. jejuni and C. coli. Genotyping of the 47 strains by PFGE after digestion with SmaI resulted in 22 subtypes. A potential correlation was found between the SmaI profiles and the 16S rRNA sequences, as a certain SmaI type only appeared in one of the two major phylogenetic groups.Campylobacter spp., principally Campylobacter jejuni subsp.jejuni, are important food- and water-borne pathogens for man [1-3]. In the present paper Campylobacter jejuni subsp. jejuni is referred to as C. jejuni and Campylobacter jejuni subsp. doylei as C. doylei. Campylobacter jejuni is frequently found in the intestinal tract in a wide variety of wild and domesticated animals, especially
%U http://www.actavetscand.com/content/50/1/10