%0 Journal Article
%T Changes in the contents of four active diterpenoids at different growth stages in Andrographis paniculata (Burm.f.) Nees (Chuanxinlian)
%A Nanthanit Pholphana
%A Nuchanart Rangkadilok
%A Jittra Saehun
%A Supachai Ritruechai
%A Jutamaad Satayavivad
%J Chinese Medicine
%D 2013
%I BioMed Central
%R 10.1186/1749-8546-8-2
%X Plants were grown in greenhouse and in field conditions, harvested at different growth stages, and separated into different organs for determination of the four active diterpenoids by an HPLC-DAD method.The most abundant diterpenoid was AP6 between seedling and vegetative stages in the greenhouse experiment (13.38 to 23.71 mg/g in 2006 and 10.67 to 24.54 mg/g in 2007). High levels of AP6 were also detected in leaves at the transfer stage in the greenhouse experiment (36.05£¿¡À£¿0.69 mg/g) and field experiment (30.59£¿¡À£¿1.39 mg/g). The levels of AP6 then decreased as plants matured. The highest content of AP4 was in cotyledons (16.65£¿¡À£¿4.48 mg/g) at the transfer stage. The highest contents of AP1 were detected in leaves at seed-forming stage in greenhouse experiment (24.72£¿¡À£¿1.89 mg/g) and vegetative stage in field experiment (43.16£¿¡À£¿0.92 mg/g). Flowers of A. paniculata contained high levels of AP1 (21.42£¿¡À£¿3.74 mg/g). AP3 and AP4 were at low levels in leaves at all growth stages.In A. paniculata, AP6 was at the highest level in leaves at transfer stage in both greenhouse and field experiments. AP1 was at the highest level in leaves at vegetative stage and seed-forming stage in field and greenhouse experiments, respectively. The contents of AP3 and AP4 in leaves were low at all growth stages.Andrographis paniculata (Burm.f.) Nees, (Acanthaceae) (known as Chuanxinlian in China) is used in Scandinavian and Asian countries to treat the common cold, fever, and diarrhea [1]. The aerial part of A. paniculata is used in traditional Chinese medicine [2]. A. paniculata is beneficial to the liver, immune system, respiratory system, and cardiovascular system, and shows anti-inflammatory, antimalarial, antidiarrheal, hypoglycemic, anti-fertility, anticancer, and anti-HIV activities [1-4]. These therapeutic activities of A. paniculata are attributed to four active diterpenoids; andrographolide (AP1), 14-deoxy-11,12-didehydroandrographolide (AP3), neoandrographolide (AP4), and 14-deo
%U http://www.cmjournal.org/content/8/1/2