%0 Journal Article
%T Determination of Plasma Homocysteine in Normal Adults by High Performance Liquid Chromatography with Fluorescence Detection<br>高效液相色谱法测定人血浆中总高半胱氨酸含量
%A Li Mingzhen
%A Wang Yuming
%A Zhu Xiashuang
%A Zheng Jiaju
%A <br>李明珍
%A 王毓明
%A 竺霞霜
%A 郑家驹
%J 色谱
%D 1999
%I 
%X A rapid, isocratic high performance liquid chromatographic(HPLc) method with fluorescence detection was used for the determination of total homocysteine (Hcy) levels in human plasma. Prior to reversed-phase HPLC analysis, the plasma thiols were derivatized with ABDF(7-fluoroben3o-2-oxa-1,3-diazole-4-sulfonamide), a thiol specific fluorogenic probe. A mobile phase consisted of 0. 1 mol/L potassium dihydrogen phosphate (pH 2. 1, containing 10% of acetonitrile) ensured base line separation of plasma Hey.The mean recovery of this method was 97. 75%. The intraday and interday coefficient of variations were 4. 49% and 9. 79%, respectively' The method is simple, sensitive,reproducible and very suitable for routine determination of plasma Hey in human.
%K high performance liquid chromatography
%K homocysteine
%K plasma<br>HPLC
%K 测定
%K 血浆
%K 高半胱氨酸
%K 人血浆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862B9906089BD7EAD67&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=38B194292C032A66&sid=85002451B65CE0D1&eid=7737D2F848706113&journal_id=1000-8713&journal_name=色谱&referenced_num=12&reference_num=1