%0 Journal Article
%T Determination of glyphosate and aminomethylphosphonic acid in rice using liquid chromatography-tandem mass spectrometry<br>液相色谱-串联质谱法测定稻米中的草甘膦和氨甲基膦酸残留
%A CAO Zhaoyun
%A MOU Renxiang
%A CHEN Mingxue
%A <br>曹赵云
%A 牟仁祥
%A 陈铭学
%J 色谱
%D 2010
%I 
%X A method was developed for the determination of glyphosate (GLY) and amino-methylphosphonic acid (AMPA) in rice using liquid chromatography tandem mass spectrometry (LC-MS/MS). The sample was extracted with water followed by a simple cleanup with a C18 solid phase extraction (SPE) cartridge, and then GLY and AMPA were derivatized using 9-fluorenylmethoxycarbonyl (FMOC-Cl) in borate buffer. The derivatives of GLY and AMPA were separated on a C18 column with gradient elution with the mobile phase of acetonitrile and 5 mmol/L ammonium acetate (pH 9), and finally detected with negative ion electrospray ionization-mass spectrometry (ESI-MS) in multiple reaction monitoring (MRM) mode. The results showed that the linearities of GLY and AMPA were in the concentration range of 0.00050 to 1.0 mg/L with the correlation coefficients of 0.9997 and 0.9999, respectively. The mean spiked recoveries of GLY and AMPA at 3 spiked levels ranged from 72.5% to 113.6% with the relative standard deviations (RSD, n=5) of 3.8%-16.2%. The limits of detection were 2.0 and 3.0 μg/kg for GLY and AMPA, respectively. This method is rapid, sensitive, and suitable for simultaneous determination of GLY and AMPA in rice.
%K pre-column derivatization
%K liquid chromatography-tandem mass spectrometry (LC-MS/MS)
%K glyphosate
%K aminomethylphosphonic acid
%K rice<br>柱前衍生化
%K 液相色谱-串联质谱
%K 草甘膦
%K 氨甲基膦酸
%K 稻米
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D99CC556BA56C57CE334F410306CD4D4&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=5D311CA918CA9A03&sid=6EDA906E07280FB0&eid=0B9C8D3D9D6254B2&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=17