%0 Journal Article
%T Advances in multidimensional high performance liquid chromatography for separation technology in proteomic study<br>多维高效液相色谱技术在蛋白质分离研究中的进展
%A GAO Mingxia
%A GUAN Xia
%A HONG Guangfeng
%A ZHANG Xiangmin
%A <br>高明霞
%A 关霞
%A 洪广峰
%A 张祥民
%J 色谱
%D 2009
%I 
%X With the developments of proteomic study, multidimensional high performance liquid chromatography technology has attracted increasing interests due to its obvious advantages, such as rapid analysis, high automation and easy combination with mass spectrometry and so on. This review emphasizes the advances of multidimensional high performance liquid chromatography technology, including classical bottom-up methods, top-down techniques and array-based two-dimensional liquid chromatography system, which was designed and set up by our lab to improve the throughput. These techniques showed promising potential applications in proteomics study.
%K multidimensional liquid chromatography
%K bottom-up
%K top-down
%K array-based two-dimensional liquid chromatography
%K intact protein
%K protein separation<br>多维液相色谱
%K bottom-up
%K top-down
%K 阵列式
%K 完整蛋白质
%K 蛋白质分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A8DA30ED06C80D5DB7A9B631A6E13131&yid=DE12191FBD62783C&vid=DB817633AA4F79B9&iid=94C357A881DFC066&sid=CEFF71AEB051114C&eid=AF4A4411BB448A36&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=35