%0 Journal Article
%T Determination of Enantiomeric Purity for Epinephrine by High Performance Liquid Chromatography<br>高效液相色谱法测定肾上腺素的光学纯度
%A Li Hua
%A Luo Wei
%A Hu Xianmin
%A <br>李桦
%A 罗维
%A 胡先明
%J 色谱
%D 1999
%I 
%X A procedure for the resolution of D,L-epinephrine and the determination of D-isomer ratio in a pharmaceutical formulation is described. The optical isomers of epinephrine were resolved by RP-HPLC with heptakis(2,6-di-O-methyl-beta-cyclodextrin) as a chiral mobile phase additives. The results showed that a correction factor must be introduced into the equation used for the calculation of the percentage of D-epinephrine, because the UV absorbance may not be the same when DM-beta-CD is present. Quantitation was achieved with external and internal standard method, HVA as an internal standard. This method can be used for the determination of the D-isomer in the L-epinephrine pharmaceutical formulation. The results showed that the mass fraction of D-isomer in the formulation increased during the period of storage.
%K high perfromance liquid chromatography
%K epinephrine
%K chiral resolution<br>光学纯度
%K 肾上腺素
%K 手性分离
%K HPLC
%K 对映体
%K 测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862C318525ABD86C6EC&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=1E9426A299DC9FFD&eid=DA74B62FE4348759&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=4