%0 Journal Article
%T Determination of Risperidone and 9-Hydroxyrisperidone in Plasma by High Performance Liquid Chromatography<br>反相高效液相色谱法测定人血浆中利培酮及其代谢物的质量浓度
%A Xiao Hong
%A Yang Jing
%A Zhang Shining
%A Zhang Xinbao
%A <br>肖红
%A 杨竟
%A 张石宁
%A 张心保
%J 色谱
%D 1999
%I 
%X A reversed-phase HPLC method for the determination of risperidone and 9-hydroxyrisperidone in plasma was established. Risperidone and 9-hydroxyrisperidone can be separated on a Zorbax ODS C18 column with a mobile phase of MeOH-water-ammonium acetate (mol/L)-ammonia water(3 mol/L) (300:50:3:1, volume ratios) and detected at 280 nm. The flow rate was 0.8 mL/min. The linear range was 2-600 micrograms/L, r = 0.996 for risperidone, and 2-800 micrograms/L, r = 0.998 for 9-hydroxyrisperidone. The average recovery was (98.2 +/- 3.5)% for risperidone, and (97.8 +/- 3.8)% for 9-hydorxyrisperidone. The intra-day and inter-day coefficients of variation were 4.12% and 4.83% for risperidone respectively, 4.28% and 4.81% for 9-hydroxyrisperidone respectively. The method has been used to determine risperidone and 9-hydroxyrisperidone mass concentration in patient plasma. The data obtained showed that the sensitivity and selectivity of this method were adequate for drug monitoring in clinical research.
%K high performance liquid chromatography
%K risperidone
%K 9-hydroxyrisperidone
%K plasma<br>利培酮
%K 羟利培酮
%K 血浆
%K HPLC
%K 测定
%K 人血浆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B8621B67118793FAD62C&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=396DD691E964F390&eid=7D6CD8918B045FD4&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=1