%0 Journal Article
%T Determination of Acyclovir in Mouse Plasma and Tissues byReversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法测定小鼠血浆及组织中的阿昔洛韦浓度
%A XU Ying
%A ZHOU Shi wen
%A TANG Jian lin
%A HUANG Lin qing
%A <br>徐颖
%A 周世文
%A 汤建林
%A 黄林清
%J 色谱
%D 2001
%I 
%X The aim of this study was to establish an high performance liquid chromatographic method for determining acyclovir (ACV) concentration in mouse plasma and tissues. A solution of 0.25 mL 60 g/L perchloric acid and 0.25 mL acetonitrile was added into 0.2 mL plasma or 0.2 g tissues to precipitate proteins. Following centrifugation, the supernatant obtained was injected into a reversed-phase column. Operating conditions were Hypersil ODS column(250 mm x 4.6 mm i.d., 5 microns), methanol-water-acetic acid(1:99:0.5, volume ratio) solution as mobile phase at a flow rate of 1.5 mL/min, UV detection at 252 nm. The detection limit of ACV concentration in plasma was 20 micrograms/L and that in tissues was 50 ng/g. The standard curves for ACV were linear in plasma and homogenate of tissues (r > 0.99). The precision of the method was good and the recoveries of ACV were higher than 97.5%. So this method is rapid, accurate and convenient for determination of ACV concentrations in plasma and tissues.
%K high performance liquid chromatography
%K acyclovir
%K mouse
%K plasma
%K tissue<br>反相高效液相色谱法
%K 阿昔洛韦
%K 血浆
%K 组织
%K 浓度测定
%K 抗病毒药物
%K 药代动力学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=7ABDD6BD56E2461E&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=B31275AF3241DB2D&sid=E49BED2EA9A8956B&eid=DFBC046213B3DD86&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=4